Isolation of single-base genome-edited human iPS cells without antibiotic selection.

ABSTRACT: Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.

SUBMITTER: Miyaoka Y 

PROVIDER: S-EPMC4063274 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature