Hydrogen sulfide deactivates common nitrobenzofurazan-based fluorescent thiol labeling reagents.

ABSTRACT: Sulfhydryl-containing compounds, including thiols and hydrogen sulfide (H2S), play important but differential roles in biological structure and function. One major challenge in separating the biological roles of thiols and H2S is developing tools to effectively separate the reactivity of these sulfhydryl-containing compounds. To address this challenge, we report the differential responses of common electrophilic fluorescent thiol labeling reagents, including nitrobenzofurazan-based scaffolds, maleimides, alkylating agents, and electrophilic aldehydes, toward cysteine and H2S. Although H2S reacted with all of the investigated scaffolds, the photophysical response to each scaffold was significantly different. Maleimide-based, alkylating, and aldehydic thiol labeling reagents provided a dimin

SUBMITTER: Montoya LA 

PROVIDER: S-EPMC4063329 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature