Choice of bacterial DNA extraction method from fecal material influences community structure as evaluated by metagenomic analysis.

ABSTRACT:

Background

In recent years, studies on the human intestinal microbiota have attracted tremendous attention. Application of next generation sequencing for mapping of bacterial phylogeny and function has opened new doors to this field of research. However, little attention has been given to the effects of choice of methodology on the output resulting from such studies.

Results

IN THIS STUDY WE CONDUCTED A SYSTEMATIC COMPARISON OF THE DNA EXTRACTION METHODS USED BY THE TWO MAJOR COLLABORATIVE EFFORTS: The European MetaHIT and the American Human Microbiome Project (HMP). Additionally, effects of homogenizing the samples before extraction were addressed. We observed significant differences in distribution of bacterial taxa depending on the method. While eukaryotic DNA was most ef

SUBMITTER: Wesolowska-Andersen A 

PROVIDER: S-EPMC4063427 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature