Dominance effects of deleterious and beneficial mutations in a single gene of the RNA virus ϕ6.

ABSTRACT: Most of our knowledge of dominance stems from studies of deleterious mutations. From these studies we know that most deleterious mutations are recessive, and that this recessivity arises from a hyperbolic relationship between protein function (i.e., protein concentration or activity) and fitness. Here we investigate whether this knowledge can be used to make predictions about the dominance of beneficial and deleterious mutations in a single gene. We employed a model system--the bacteriophage φ6--that allowed us to generate a collection of mutations in haploid conditions so that it was not biased toward either dominant beneficial or recessive deleterious mutations. Screening for the ability to infect a bacterial host that does not permit infection by the wildtype φ6, we generated a collecti

SUBMITTER: Joseph SB 

PROVIDER: S-EPMC4063744 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature