Reconstituted human upper airway epithelium as 3-d in vitro model for nasal polyposis.

ABSTRACT:

Background

Primary human airway epithelial cells cultured in an air-liquid interface (ALI) develop a well-differentiated epithelium. However, neither characterization of mucociliar differentiation overtime nor the inflammatory function of reconstituted nasal polyp (NP) epithelia have been described.

Objectives

1st) To develop and characterize the mucociliar differentiation overtime of human epithelial cells of chronic rhinosinusitis with nasal polyps (CRSwNP) in ALI culture system; 2nd) To corroborate that 3D in vitro model of NP reconstituted epithelium maintains, compared to control nasal mucosa (NM), an inflammatory function.

Methods

Epithelial cells were obtained from 9 NP and 7 control NM, and differentiated in ALI culture for 28 days. Mucociliary differentiation

SUBMITTER: de Borja Callejas F 

PROVIDER: S-EPMC4063947 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature