Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes.

ABSTRACT: The opportunistic fungal pathogen Candida glabrata is a frequent cause of candidiasis, causing infections ranging from superficial to life-threatening disseminated disease. The inherent tolerance of C. glabrata to azole drugs makes this pathogen a serious clinical threat. To identify novel genes implicated in antifungal drug tolerance, we have constructed a large-scale C. glabrata deletion library consisting of 619 unique, individually bar-coded mutant strains, each lacking one specific gene, all together representing almost 12% of the genome. Functional analysis of this library in a series of phenotypic and fitness assays identified numerous genes required for growth of C. glabrata under normal or specific stress conditions, as well as a number of novel genes involved in tolerance to clin

SUBMITTER: Schwarzmuller T 

PROVIDER: S-EPMC4063973 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature