A highly functional synthetic phage display library containing over 40 billion human antibody clones.

ABSTRACT: Several synthetic antibody phage display libraries have been created and used for the isolation of human monoclonal antibodies. The performance of antibody libraries, which is usually measured in terms of their ability to yield high-affinity binding specificities against target proteins of interest, depends both on technical aspects (such as library size and quality of cloning) and on design features (which influence the percentage of functional clones in the library and their ability to be used for practical applications). Here, we describe the design, construction and characterization of a combinatorial phage display library, comprising over 40 billion human antibody clones in single-chain fragment variable (scFv) format. The library was designed with the aim to obtain highly stable anti

SUBMITTER: Weber M 

PROVIDER: S-EPMC4065035 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature