A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes.

ABSTRACT:

Background

Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from other species can, however, also be used. Because of the way in which gene conversion introduces diversity, the naïve antibody repertoire of the chicken can easily be accessed using only two sets of primers.

Results

With in vitro diagnostic applications in mind, we have constructed a large library of recombinant filamentous bacteriophages displaying single chain antibody fragments derived from combinatorial pairings of chicken variable heavy and light chains. Synthetically randomised complementarity determining regions are included in some of the heavy chains.

SUBMITTER: van Wyngaardt W 

PROVIDER: S-EPMC406508 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature