Leukocyte-adjusted epigenome-wide association studies of blood from solid tumor patients.

ABSTRACT: Epigenome-wide studies of DNA methylation using blood-derived DNA from cancer patients are complicated by the heterogeneity of cell types within blood and the associated cell lineage specification of DNA methylation signatures. Here, we applied a novel set of analytic approaches to assess the association between cancer case-status and DNA methylation adjusted for leukocyte variation using blood specimens from three case-control cancer studies (bladder: 223 cases, 205 controls; head and neck: 92 cases, 92 controls; and ovarian: 131 cases, 274 controls). Using previously published data on leukocyte-specific CpG loci and a recently described approach to deconvolute subject-specific blood composition, we performed an epigenome-wide analysis to examine the association between blood-based DNA me

SUBMITTER: Langevin SM 

PROVIDER: S-EPMC4065186 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature