Influence of CHIEF pathway genes on gene expression: a pathway approach to functionality.

ABSTRACT:

Background

Candidate pathway approaches in disease association studies often utilize a tagSNP approach to capture genetic variation. In this paper we assess gene expression patterns with SNPs in genes in the CHIEF pathway to help determine their potential functionality.

Methods

Quantitative real-time RT-PCR was run to determine gene expression of 13 genes in normal colon tissue samples from 82 individuals. TagSNP genotype data were obtained from a GoldenGate Illumina multiplex bead array platform. Age, sex, and genetic ancestry adjusted general linear models were used to estimate beta coefficients and p values.

Results

Genetic variation in mTOR (1 SNP), NFKB1 (4 SNPs), PRKAG2 (3 SNPs), and TSC2 (1 SNP) significantly influenced their expression. After adjustment for mu

SUBMITTER: Slattery ML 

PROVIDER: S-EPMC4065398 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature