Identification of the differential expressive tumor associated genes in rectal cancers by cDNA microarray.

ABSTRACT:

Aim

To identify tumor associated genes of rectal cancer and to probe the application possibility of gene expression profiles for the classification of tumors.

Methods

Rectal cancer tissues and their paired normal mucosa were obtained from patients undergoing surgical resection of rectal cancer. Total RNA was extracted using Trizol reagents. First strand cDNA synthesis was indirectly labeled with aminoallyl-dUTP and coupled with Cy3 or Cy5 dye NHS mono-functional ester. After normalization to total spots, the genes which background subtracted intensity did not exceed 2 SD above the mean blank were excluded. The data were then sorted to obtain genes differentially expressed by >or= 2 fold up or down in at least 5 of the 21 patients.

Results

In the 21 rectal cancer patie

SUBMITTER: Gao XQ 

PROVIDER: S-EPMC4065886 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature