Role of Kupffer cells in acute hemorrhagic necrotizing pancreatitis-associated lung injury of rats.

ABSTRACT:

Aim

To investigate the role of Kupffer cells (KCs) in acute hemorrhagic necrotizing pancreatitis-associated lung injury (AHNP-LI).

Methods

Forty-two rats were allocated to four groups [sham operation, AHNP model, gadolinium chloride (GdCl3) pretreatment, GdCl3 control]. In GdCl3 pretreatment group, GdCl3 was administered by caudal vein injection 24 h before the AHNP model induction. Blood from the iliac artery, alveolar macrophages and tissues from the pancreas and lung, were collected in six animals per group 3 and 6 h after acute pancreatitis induction. TNF-alpha, IL-1 of serum, myeloperoxidase (MPO) of lung tissue, NF-kappaB activation of alveolar macrophages were detected. Serum AST and ALT in sham operation group and GdCl3 control group were tested. In addition, histopa

SUBMITTER: Liu HB 

PROVIDER: S-EPMC4066058 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature