Dataset Information

Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women.

ABSTRACT:

Objective

Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS).

Methods

Setting

Center for Translational Science Activities (CTSA).

Participants

Healthy nonpregnant women (N=120) ages 21 to 79 years.

Outcomes

SHBG, testosterone (T), estradiol (E2) and estrone (E1) each determined by MS. Uni- and multivariate regression of SHBG concentrations on age, body mass index (BMI), total and visceral abdominal fat (TAF, AVF), albumin, glucose, insulin, sex steroids, selected cytokines, blood pressure, and lipids.

Results

By univariate regression, MS-estimated SHBG correlated negatively with BMI, TAF, AVF, insulin, free T and bioavailable T (bio T) (each P≤10(-4)), but not with blood pressure or lipids. By stepwise multivariate regression analysis, free and total T (both positive) and bio T (negative) were correlated with SHBG in all 4 assays (each P<10(-15), R(2)≥0.481). In addition, TAF and BMI were negatively associated with SHBG (P≤0.0066) in 2 SHBG assays, and estrone and IL-8 with SHBG weakly (P≤0.035) in one SHBG assay each. When nonsignificant cytokines were excluded, SHBG was jointly associated with AVF, total T and HDL (P<10(-9), R(2)=0.358).

Conclusion

According to MS, three metabolic factors, T, AVF and HDL, together explain more than one-third of the interindividual variation in SHBG levels. We speculate that these measures reflect insulin action.

SUBMITTER: Veldhuis JD

PROVIDER: S-EPMC4066221 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women.

Veldhuis Johannes D JD Dyer Roy B RB Trushin Sergey A SA Bondar Olga P OP Singh Ravinder J RJ Klee George G GG

Metabolism: clinical and experimental 20140321 6

<h4>Objective</h4>Sex-hormone binding globulin (SHBG) concentrations across the adult female lifespan are not well defined. To address this knowledge gap, SHBG was quantified by both immunological and criterion methods, viz, mass spectrometry (MS).<h4>Methods</h4><h4>Setting</h4>Center for Translational Science Activities (CTSA).<h4>Participants</h4>Healthy nonpregnant women (N=120) ages 21 to 79 years.<h4>Outcomes</h4>SHBG, testosterone (T), estradiol (E2) and estrone (E1) each determined by MS ...[more]

PMID: 24746136

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Dataset Information

Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women.

Objective

Methods

Setting

Participants

Outcomes

Results

Conclusion

Publications

Immunologic and mass-spectrometric estimates of SHBG concentrations in healthy women.

Similar Datasets

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