Functional exofacially tagged N-type calcium channels elucidate the interaction with auxiliary α2δ-1 subunits.

ABSTRACT: CaV1 and CaV2 voltage-gated calcium channels are associated with β and α2δ accessory subunits. However, examination of cell surface-associated CaV2 channels has been hampered by the lack of antibodies to cell surface-accessible epitopes and of functional exofacially tagged CaV2 channels. Here we report the development of fully functional CaV2.2 constructs containing inserted surface-accessible exofacial tags, which allow visualization of only those channels at the plasma membrane, in both a neuronal cell line and neurons. We first examined the effect of the auxiliary subunits. Although α2δ subunits copurify with CaV2 channels, it has recently been suggested that this interaction is easily disrupted and nonquantitative. We have now tested whether α2δ subunits are associated with these chann

SUBMITTER: Cassidy JS 

PROVIDER: S-EPMC4066493 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature