Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera).

ABSTRACT: Honeybees (Apis mellifera), which are important pollinators of plants, display remarkable individual behaviors that collectively contribute to the organization of a complex society. Advances in dissecting the complex processes of honeybee behavior have been limited in the recent past due to a lack of genetic manipulation tools. These tools are difficult to apply in honeybees because the unit of reproduction is the colony, and many interesting phenotypes are developmentally specified at later stages. Here, we report highly efficient integration and expression of piggyBac-derived cassettes in the honeybee. We demonstrate that 27 and 20% of queens stably transmitted two different expression cassettes to their offspring, which is a 6- to 30-fold increase in efficiency compared with those gener

SUBMITTER: Schulte C 

PROVIDER: S-EPMC4066538 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature