Fluorescence-enabled electrochemical microscopy with dihydroresorufin as a fluorogenic indicator.

ABSTRACT: Recently, we introduced a new electrochemical imaging technique called fluorescence-enabled electrochemical microscopy (FFEM). The central idea of FEEM is that a closed bipolar electrode is utilized to electrically couple a redox reaction of interest to a complementary fluorogenic reaction converting an electrochemical signal into a fluorescent signal. This simple strategy enables one to use fluorescence microscopy to observe conventional electrochemical processes on very large electrochemical arrays. The initial demonstration of FEEM focused on the use of a specific fluorogenic indicator, resazurin, which is reduced to generate highly fluorescent resorufin. The use of resazurin has enabled the study of analyte oxidation reactions, such as the oxidation of dopamine and H2O2. In this report

SUBMITTER: Oja SM 

PROVIDER: S-EPMC4066899 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature