Correlation between AcrB trimer association affinity and efflux activity.

ABSTRACT: The majority of membrane proteins function as oligomers. However, it remains largely unclear how the oligomer stability of protein complexes correlates with their function. Understanding the relationship between oligomer stability and activity is essential to protein research and to virtually all cellular processes that depend on the function of protein complexes. Proteins make lasting or transient interactions as they perform their functions. Obligate oligomeric proteins exist and function exclusively at a specific oligomeric state. Although oligomerization is clearly critical for such proteins to function, a direct correlation between oligomer affinity and biological activity has not yet been reported. Here, we used an obligate trimeric membrane transporter protein, AcrB, as a model to i

SUBMITTER: Ye C 

PROVIDER: S-EPMC4067148 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature