Novel transcripts from a distinct promoter that encode the full-length AKT1 in human breast cancer cells.

ABSTRACT:

Background

The serine-threonine kinase AKT1 plays essential roles during normal mammary gland development as well as the initiation and progression of breast cancer. AKT1 is generally considered a ubiquitously expressed gene, and its persistent activation is transcriptionally controlled by regulatory elements characteristic of housekeeping gene promoters. We recently identified a novel Akt1 transcript in mice (Akt1m), which is induced by growth factors and their signal transducers of transcription from a previously unknown promoter. The purpose of this study was to examine whether normal and neoplastic human breast epithelial cells express an orthologous AKT1m transcript and whether its expression is deregulated in cancer cells.

Methods

Initial sequence analyses were perform

SUBMITTER: Schmidt JW 

PROVIDER: S-EPMC4068023 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature