In vivo assessment of drug efficacy against Mycobacterium abscessus using the embryonic zebrafish test system.

ABSTRACT: Mycobacterium abscessus is responsible for a wide spectrum of clinical syndromes and is one of the most intrinsically drug-resistant mycobacterial species. Recent evaluation of the in vivo therapeutic efficacy of the few potentially active antibiotics against M. abscessus was essentially performed using immunocompromised mice. Herein, we assessed the feasibility and sensitivity of fluorescence imaging for monitoring the in vivo activity of drugs against acute M. abscessus infection using zebrafish embryos. A protocol was developed where clarithromycin and imipenem were directly added to water containing fluorescent M. abscessus-infected embryos in a 96-well plate format. The status of the infection with increasing drug concentrations was visualized on a spatiotemporal level. Drug efficacy

SUBMITTER: Bernut A 

PROVIDER: S-EPMC4068527 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature