Application of next generation sequencing to CEPH cell lines to discover variants associated with FDA approved chemotherapeutics.

ABSTRACT:

Background

The goal of this study was to perform candidate gene association with cytotoxicity of chemotherapeutics in cell line models through resequencing and discovery of rare and low frequency variants along with common variations. Here, an association study of cytotoxicity response to 30 FDA approved drugs was conducted and we applied next generation targeted sequencing technology to discover variants from 103 candidate genes in 95 lymphoblastoid cell lines from 14 CEPH pedigrees. In this article, we called variants across 95 cell lines and performed association analysis for cytotoxic response using the Family Based Association Testing method and software.

Results

We called 2281 variable SNP genotypes across the 103 genes for these cell lines and identified three genes o

SUBMITTER: Hariani GD 

PROVIDER: S-EPMC4068968 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature