Variability of CSF Alzheimer's disease biomarkers: implications for clinical practice.

ABSTRACT:

Background

Cerebrospinal fluid (CSF) biomarkers are increasingly being used for diagnosis of Alzheimer's disease (AD).

Objective

We investigated the influence of CSF intralaboratory and interlaboratory variability on diagnostic CSF-based AD classification of subjects and identified causes of this variation.

Methods

We measured CSF amyloid-β (Aβ) 1-42, total tau (t-tau), and phosphorylated tau (p-tau) by INNOTEST enzyme-linked-immunosorbent assays (ELISA) in a memory clinic population (n = 126). Samples were measured twice in a single or two laboratories that served as reference labs for CSF analyses in the Netherlands. Predefined cut-offs were used to classify CSF biomarkers as normal or abnormal/AD pattern.

Results

CSF intralaboratory variability was higher fo

SUBMITTER: Vos SJ 

PROVIDER: S-EPMC4069102 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature