Rapid adaptive optical recovery of optimal resolution over large volumes.

ABSTRACT: Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

SUBMITTER: Wang K 

PROVIDER: S-EPMC4069208 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature