Pax3 isoforms in sensory neurogenesis: expression and function in the ophthalmic trigeminal placode.

ABSTRACT:

Background

In the trigeminal placode, Pax3 is classified as necessary but not sufficient for sensory neuron differentiation. One hypothesis is that different Pax3 isoforms regulate cellular differentiation uniquely. Pax3 is known to sometimes activate and sometimes repress gene transcription, and its activity can be dependent on the isoforms present. Pax3 isoforms had not previously been characterized in chick sensory neurogenesis.

Results

Reverse transcriptase-polymerase chain reaction (PCR) analysis revealed three well-expressed Pax3 splice variants: full-length (flPax3), Pax3V1, and Pax3V2. Each was characterized for its effect on neurogenesis by misexpression in placodal ectoderm. The differences observed were more apparent under conditions of enhanced neurogenesis (by m

SUBMITTER: Adams JS 

PROVIDER: S-EPMC4069251 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature