Seroprevalence and evolutionary dynamics of genotype 4 hepatitis E virus in Shandong Province, China.

ABSTRACT:

Aim

To investigate the seroprevalence and evolutionary dynamics of hepatitis E virus (HEV) and assess the ancestor of HEVs in China's Shandong Province.

Methods

A total of 2028 serum, 60 fecal and 82 bile samples were collected from the general human population, patients and swine, respectively. This seroepidemiological study was conducted using an immunnosorbent assay and HEV RNA was detected by the reverse transcription-nested polymerase chain reaction (RT-nPCR) method. Complete genome sequences of the prevalent strains (CH-YT-HEV01, CH-YT-HEV02 and CH-YT-sHEV01) were determined, and the sequences were analyzed phylogenetically. In addition, the evolutionary dynamics of three HEV isolates were determined using the framework of coalescent analysis in the program package BEA

SUBMITTER: Yang D 

PROVIDER: S-EPMC4069323 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature