Synthetic promoter library for modulation of actinorhodin production in Streptomyces coelicolor A3(2).

ABSTRACT: The objective of this study was the application of the synthetic promoter library (SPL) technology for modulation of actinorhodin production in Streptomyces coelicolor A3(2). The SPL technology was used to optimize the expression of a pathway specific positive transcriptional regulator ActII orf4, which activates the transcription of the S. coelicolor actinorhodin biosynthetic gene cluster. The native actII orf4 promoter was replaced with synthetic promoters, generating a S. coelicolor library with a broad range of expression levels of actII orf4. The resulting library was screened based on the yield of actinorhodin. Selected strains were further physiologically characterized. One of the strains from the library, ScoSPL20, showed considerably higher yield of actinorhodin and final actinorh

SUBMITTER: Sohoni SV 

PROVIDER: S-EPMC4070896 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature