Characterization of p38 MAPK isoforms for drug resistance study using systems biology approach.

ABSTRACT:

Motivation

p38 mitogen-activated protein kinase activation plays an important role in resistance to chemotherapeutic cytotoxic drugs in treating multiple myeloma (MM). However, how the p38 mitogen-activated protein kinase signaling pathway is involved in drug resistance, in particular the roles that the various p38 isoforms play, remains largely unknown.

Method

To explore the underlying mechanisms, we developed a novel systems biology approach by integrating liquid chromatography-mass spectrometry and reverse phase protein array data from human MM cell lines with computational pathway models in which the unknown parameters were inferred using a proposed novel algorithm called modularized factor graph.

Results

New mechanisms predicted by our models suggest that combine

SUBMITTER: Peng H 

PROVIDER: S-EPMC4071201 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature