MicroRNA-26a regulates glucose metabolism by direct targeting PDHX in colorectal cancer cells.

ABSTRACT:

Background

Reprogramming energy metabolism has been an emerging hallmark of cancer cells. MicroRNAs play important roles in glucose metabolism.

Methods

The targets of microRNA-26a (miR-26a) were predicted by bioinformatics tools. The efficacy of miR-26a binding the 3'-untranslated region (UTR) of pyruvate dehydrogenase protein X component (PDHX) mRNA was evaluated using a dual-luciferase reporter assay. The PDHX expression at the mRNA and protein level in several colon cancer cell lines was quantified with real-time PCR and Western blot analysis respectively. The effects of miR-26a on glucose metabolism were determined by detecting the content of glucose consumption, production of lactate, pyruvate, and acetyl-coenzyme A.

Results

The expression of miR-26a is inversely

SUBMITTER: Chen B 

PROVIDER: S-EPMC4071217 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature