Fusion, rupture, and degeneration: the fate of in vivo-labelled PSVs in developing barley endosperm.

ABSTRACT: Cereal endosperm is a highly differentiated tissue containing specialized organelles for the accumulation of storage proteins. The endosperm of barley contains hordeins, which are ultimately deposited within protein storage vacuoles (PSVs). These organelles have been characterized predominantly by the histochemical analysis of fixed immature tissue samples. However, little is known about the fate of PSVs during barley endosperm development, and in vivo imaging has not been attempted in order to gain further insight. In this report, young seeds were followed through development to characterize the dynamic morphology of PSVs from aleurone, subaleurone, and central starchy endosperm cells. TIP3-GFP was used as a PSV membrane marker and several fluorescent tracers were used to identify membran

SUBMITTER: Ibl V 

PROVIDER: S-EPMC4071841 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature