Analysis of N-glycoproteins using genomic N-glycosite prediction.

ABSTRACT: Protein glycosylation has long been recognized as one of the most common post-translational modifications. Most membrane proteins and extracellular proteins are N-linked glycosylated, and they account for the majority of current clinical diagnostic markers or therapeutic targets. Quantitative proteomic analysis of detectable N-linked glycoproteins from cells or tissues using mass spectrometry has the potential to provide biological basis for disease development and identify disease associated glycoproteins. However, the information of low abundance but important peptides is lost due to the lack of MS/MS fragmentation or low quality of MS/MS spectra for low abundance peptides. Here, we show the feasibility of formerly N-glycopeptide identification and quantification at MS1 level using genom

SUBMITTER: Sun S 

PROVIDER: S-EPMC4072220 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature