Yeast mating and image-based quantification of spatial pattern formation.

ABSTRACT: Communication between cells is a ubiquitous feature of cell populations and is frequently realized by secretion and detection of signaling molecules. Direct visualization of the resulting complex gradients between secreting and receiving cells is often impossible due to the small size of diffusing molecules and because such visualization requires experimental perturbations such as attachment of fluorescent markers, which can change diffusion properties. We designed a method to estimate such extracellular concentration profiles in vivo by using spatiotemporal mathematical models derived from microscopic analysis. This method is applied to populations of thousands of haploid yeast cells during mating in order to quantify the extracellular distributions of the pheromone α-factor and the activ

SUBMITTER: Diener C 

PROVIDER: S-EPMC4072512 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature