The use of massive sequencing to detect differences between immature embryos of MON810 and a comparable non-GM maize variety.

ABSTRACT: The insect resistant maize YieldGard MON810 was studied to assess the extent to which introduction of a transgene may putatively alter the expression of endogenous genes by comparison of various GM lines vs. their non-transgenic counterparts. To assess the extent to which introduction of a transgene may putatively alter the expression of endogenous genes, GM lines of the insect resistant maize YieldGard MON810 were compared with non-transgenic counterparts. For a more in-depth study, high-throughput deep sequencing together with microarrays were used to compare the transcriptomes of immature embryos of the MON810 variety DKC6575, with a cryIA(b) transgene, and its near-isogenic variety Tietar, grown under controlled environmental conditions. This technique also allows characterisation of t

SUBMITTER: La Paz JL 

PROVIDER: S-EPMC4072715 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature