Influence of handling conditions on the establishment and propagation of head and neck cancer patient derived xenografts.

ABSTRACT:

Background

Patient derived xenografts (PDXs) for head and neck cancer (HNC) and other cancers represent powerful research platforms. Most groups implant patient tissue into immunodeficient mice immediately although the significance of this time interval is anecdotal. We tested the hypothesis that the time from tumor excision to implantation is crucial for PDX passaging and establishment.

Methods

We examined whether time or storage medium affected PDX viability for passaging two established HNC PDXs (UW-SCC34, UW-SCC52). Tumors were harvested, stored in ice-cold media or saline for 0-48 hours, and implanted into new mice. Tumor growth was compared by two-way ANOVA with respect to time and storage condition. Three new HNC PDXs (UW-SCC63-65) were generated by implanting patient

SUBMITTER: Stein AP 

PROVIDER: S-EPMC4072729 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature