Chronic cocaine-regulated epigenomic changes in mouse nucleus accumbens.

ABSTRACT:

Background

Increasing evidence supports a role for altered gene expression in mediating the lasting effects of cocaine on the brain, and recent work has demonstrated the involvement of chromatin modifications in these alterations. However, all such studies to date have been restricted by their reliance on microarray technologies that have intrinsic limitations.

Results

We use next generation sequencing methods, RNA-seq and ChIP-seq for RNA polymerase II and several histone methylation marks, to obtain a more complete view of cocaine-induced changes in gene expression and associated adaptations in numerous modes of chromatin regulation in the mouse nucleus accumbens, a key brain reward region. We demonstrate an unexpectedly large number of pre-mRNA splicing alterations in res

SUBMITTER: Feng J 

PROVIDER: S-EPMC4073058 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature