Intravital correlated microscopy reveals differential macrophage and microglial dynamics during resolution of neuroinflammation.

ABSTRACT: Many brain diseases involve activation of resident and peripheral immune cells to clear damaged and dying neurons. Which immune cells respond in what way to cues related to brain disease, however, remains poorly understood. To elucidate these in vivo immunological events in response to brain cell death we used genetically targeted cell ablation in zebrafish. Using intravital microscopy and large-scale electron microscopy, we defined the kinetics and nature of immune responses immediately following injury. Initially, clearance of dead cells occurs by mononuclear phagocytes, including resident microglia and macrophages of peripheral origin, whereas amoeboid microglia are exclusively involved at a later stage. Granulocytes, on the other hand, do not migrate towards the injury. Remarkably, fol

SUBMITTER: van Ham TJ 

PROVIDER: S-EPMC4073275 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature