A serine cluster mediates BMAL1-dependent CLOCK phosphorylation and degradation.

ABSTRACT: The circadian clock regulates biological processes from gene expression to organism behavior in a precise, sustained rhythm that is generated at the unicellular level by coordinated function of interlocked transcriptional feedback loops and post-translational modifications of core clock proteins. CLOCK phosphorylation regulates transcriptional activity, cellular localization and stability; however little is known about the specific residues and enzymes involved. We have identified a conserved cluster of serines that include, Ser431, which is a prerequisite phosphorylation site for the generation of BMAL dependent phospho-primed CLOCK and for the potential GSK-3 phosphorylation at Ser427. Mutational analysis and protein stability assays indicate that this serine cluster functions as a phosp

SUBMITTER: Spengler ML 

PROVIDER: S-EPMC4073639 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature