Directed differentiation of human induced pluripotent stem cells toward bone and cartilage: in vitro versus in vivo assays.

ABSTRACT: The ability to differentiate induced pluripotent stem cells (iPSCs) into committed skeletal progenitors could allow for an unlimited autologous supply of such cells for therapeutic uses; therefore, we attempted to create novel bone-forming cells from human iPSCs using lines from two distinct tissue sources and methods of differentiation that we previously devised for osteogenic differentiation of human embryonic stem cells, and as suggested by other publications. The resulting cells were assayed using in vitro methods, and the results were compared with those obtained from in vivo transplantation assays. Our results show that true bone was formed in vivo by derivatives of several iPSC lines, but that the successful cell lines and differentiation methodologies were not predicted by the resu

SUBMITTER: Phillips MD 

PROVIDER: S-EPMC4073820 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature