Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes.

ABSTRACT:

Background

Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown.

Results

In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell-like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of t

SUBMITTER: Zhou D 

PROVIDER: S-EPMC4074394 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature