Acute exposure of methylglyoxal leads to activation of KATP channels expressed in HEK293 cells.

ABSTRACT:

Aim

Highly reactive carbonyl methylglyoxal (MGO) is one of the metabolites excessively produced in diabetes. We have showed that prolonged exposure of vascular smooth muscle cells to MGO leads to instability of the mRNA encoding ATP-sensitive potassium (KATP) channel. In the present study we investigated the effects of MGO on the activity of KATP channels.

Methods

Kir6.1/ SUR2B, Kir6.2/SUR2B or Kir6.2Δ36 (a truncated Kir6.2 isoform) alone was expressed in HEK293 cells. Whole-cell currents were recorded in the cells with an Axopatch 200B amplifier. Macroscopic currents and single-channel currents were recorded in giant inside-out patches and normal inside-out patches, respectively. Data were analyzed using Clampfit 9 software.

Results

The basal activity of Kir6.1/SUR2B

SUBMITTER: Yang Y 

PROVIDER: S-EPMC4075750 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature