An empirical approach for quantifying loop-mediated isothermal amplification (LAMP) using Escherichia coli as a model system.

ABSTRACT: Loop mediated isothermal amplification (LAMP) is a highly efficient, selective and rapid DNA amplification technique for genetic screening of pathogens. However, despite its popularity, there is yet no mathematical model to quantify the outcome and no well-defined metric for comparing results that are available. LAMP is intrinsically complex and involves multiple pathways for gene replication, making fundamental modelling nearly intractable. To circumvent this difficulty, an alternate, empirical model is introduced that will allow one to extract a set of parameters from the concentration versus time curves. A simple recipe to deduce the time to positive, Tp--a parameter analogous to the threshold cycling time in polymerase chain reaction (PCR), is also provided. These parameters can be reg

SUBMITTER: Subramanian S 

PROVIDER: S-EPMC4076223 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature