Suitable transfection methods for single particle tracing in plant suspension cells.

ABSTRACT:

Background

A multitude of different imaging systems are already available to image genetically altered RNA species; however, only a few of these techniques are actually suitable to visualize endogenous RNA. One possibility is to use fluorescently-labelled and hybridization-sensitive probes. In order to yield more information about the exact localization and movement of a single RNA molecule, it is necessary to image such probes with highly sensitive microscope setups. More challenges arise if such experiments are conducted in plant cells due to their high autofluorescence and demanding transfection procedures.

Results

Here, we report in planta imaging of single RNA molecules using fluorescently labeled molecular beacons. We tested three different transfection protocols in or

SUBMITTER: Gohring J 

PROVIDER: S-EPMC4076440 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature