Calmodulation meta-analysis: predicting calmodulin binding via canonical motif clustering.

ABSTRACT: The calcium-binding protein calmodulin (CaM) directly binds to membrane transport proteins to modulate their function in response to changes in intracellular calcium concentrations. Because CaM recognizes and binds to a wide variety of target sequences, identifying CaM-binding sites is difficult, requiring intensive sequence gazing and extensive biochemical analysis. Here, we describe a straightforward computational script that rapidly identifies canonical CaM-binding motifs within an amino acid sequence. Analysis of the target sequences from high resolution CaM-peptide structures using this script revealed that CaM often binds to sequences that have multiple overlapping canonical CaM-binding motifs. The addition of a positive charge discriminator to this meta-analysis resulted in a tool t

SUBMITTER: Mruk K 

PROVIDER: S-EPMC4076516 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature