Isolation and kinetic characterisation of hydrophobically distinct populations of form I Rubisco.

ABSTRACT:

Background

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) is a Calvin Cycle enzyme involved in CO2 assimilation. It is thought to be a major cause of photosynthetic inefficiency, suffering from both a slow catalytic rate and lack of specificity due to a competing reaction with oxygen. Revealing and understanding the engineering rules that dictate Rubisco's activity could have a significant impact on photosynthetic efficiency and crop yield.

Results

This paper describes the purification and characterisation of a number of hydrophobically distinct populations of Rubisco from both Spinacia oleracea and Brassica oleracea extracts. The populations were obtained using a novel and rapid purification protocol that employs hydrophobic interaction chromatography (HIC) as a

SUBMITTER: O'Donnelly K 

PROVIDER: S-EPMC4076768 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature