Spatial proteomic and phospho-proteomic organization in three prototypical cell migration modes.

ABSTRACT:

Background

Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for localized membrane protrusion that drives directed cell migration. Different ensembles of proteins are therefore likely to get recruited and phosphorylated in membrane protrusions in response to specific cues.

Results

HERE, WE USE AN ASSAY THAT ALLOWS TO BIOCHEMICALLY PURIFY EXTENDING PROTRUSIONS OF CELLS MIGRATING IN RESPONSE TO THREE PROTOTYPICAL RECEPTORS: integrins, recepor tyrosine kinases and G-coupled protein receptors. Using quantitative proteomics and phospho-proteomics approaches, we provide evidence for the existence of cue-specific, spatially distinct protein networks in the different cell migration modes.

Conclusions

The integrated analysis of the large-scale

SUBMITTER: Fengos G 

PROVIDER: S-EPMC4077045 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature