Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids.

ABSTRACT: Endogenous peptides that represent biological and pathological information of disease have attracted interest for diagnosis. However, the extraction of those low abundance peptides is still a challenge because of the complexity of human bodily fluids (HBF). Hepcidin, a peptide hormone, has been recognized as a biomarker for iron-related diseases. There is no rapid and reliable way to enrich them from HBF. Here we describe a peptide extraction approach based on nanoporous silica thin films to successfully detect hepcidin from HBF. Cooperative functions of nanopore to biomolecule, including capillary adsorption, size-exclusion and electrostatic interaction, were systematically investigated to immobilize the target peptide. To promote this new approach to clinical practices, we further applie

SUBMITTER: Fan J 

PROVIDER: S-EPMC4077980 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature