Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803.

ABSTRACT:

Background

Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by "designer microbes." The chemical commodity product L-lactic acid can be synthesized in one step from a key intermediary metabolite of these organisms, pyruvate, catalyzed by a lactate dehydrogenase. Synthetic biology engineering to make "designer microbes" includes the introduction and overexpression of the product-forming biochemical pathway. For further optimization of product formation, modifications in the surrounding biochemical network of intermediary metabolism have to be made.

Results

To improve light-driven L-lactic acid production from CO2, we explored several metabolic eng

SUBMITTER: Angermayr SA 

PROVIDER: S-EPMC4078008 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature