Th17/Treg ratio derived using DNA methylation analysis is associated with the late phase asthmatic response.

ABSTRACT:

Background

The imbalance between Th17 and Treg cells has been studied in various diseases including allergic asthma but their roles have not been fully understood in the development of the late phase asthmatic response.

Objectives

To determine changes in Th17 and Treg cell numbers between isolated early responders (ERs) and dual responders (DRs) undergoing allergen inhalation challenge. To identify gene expression profiles associated with Th17 and Treg cells.

Methods

14 participants (8 ERs and 6 DRs) with mild allergic asthma underwent allergen inhalation challenge. Peripheral blood was collected prior to and 2 hours post allergen challenge. DNA methylation analysis was used to quantifiy the relative frequencies of Th17, Tregs, total B cells, and total T cells. Gene e

SUBMITTER: Singh A 

PROVIDER: S-EPMC4078401 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature