Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens.

ABSTRACT: Understanding the mechanisms by which compounds discovered using cell-based phenotypic screening strategies might exert their effects would be highly augmented by new approaches exploring their potential interactions with the genome. For example, altered androgen receptor (AR) transcriptional programs, including castration resistance and subsequent chromosomal translocations, play key roles in prostate cancer pathological progression, making the quest for identification of new therapeutic agents and an understanding of their actions a continued priority. Here we report an approach that has permitted us to uncover the sites and mechanisms of action of a drug, referred to as "SD70," initially identified by phenotypic screening for inhibitors of ligand and genotoxic stress-induced translocati

SUBMITTER: Jin C 

PROVIDER: S-EPMC4078819 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature