MALDI-Ion Mobility Mass Spectrometry of Lipids in Negative Ion Mode.

ABSTRACT: Profiling and imaging MALDI mass spectrometry (MS) allows detection and localization of biomolecules in tissue, of which lipids are a major component. However, due to the in situ nature of this technique, complexity of tissue and need for a chemical matrix, the recorded signal is complex and can be difficult to assign. Ion mobility adds a dimension that provides coarse shape information, separating isobaric lipids, peptides, and oligonucleotides along distinct familial trend lines before mass analysis. Previous work using MALDI-ion mobility mass spectrometry to analyze and image lipids has been conducted mainly in positive ion mode, although several lipid classes ionize preferentially in negative ion mode. This work highlights recent data acquired in negative ion mode to detect glyc

SUBMITTER: Jackson SN 

PROVIDER: S-EPMC4078893 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature