Coupling isotachophoresis with affinity chromatography for rapid and selective purification with high column utilization, part 1: theory.

ABSTRACT: We present a novel technique that couples isotachophoresis (ITP) with affinity chromatography (AC) to achieve rapid, selective purification with high column utilization. ITP simultaneously preconcentrates an analyte and purifies it, based on differences in mobility of sample components, excluding species that may foul or compete with the target at the affinity substrate. ITP preconcentration accelerates the affinity reaction, reducing assay time, improving column utilization, and allowing for capture of targets with higher dissociation constants. Furthermore, ITP-AC separates the target and contaminants into nondiffusing zones, thus achieving high resolution in a short distance and time. We present an analytical model for spatiotemporal dynamics of ITP-AC. We identify and explore the effec

SUBMITTER: Shkolnikov V 

PROVIDER: S-EPMC4079320 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature