Automated quantification and integrative analysis of 2D and 3D mitochondrial shape and network properties.

ABSTRACT: Mitochondrial morphology and function are coupled in healthy cells, during pathological conditions and (adaptation to) endogenous and exogenous stress. In this sense mitochondrial shape can range from small globular compartments to complex filamentous networks, even within the same cell. Understanding how mitochondrial morphological changes (i.e. "mitochondrial dynamics") are linked to cellular (patho) physiology is currently the subject of intense study and requires detailed quantitative information. During the last decade, various computational approaches have been developed for automated 2-dimensional (2D) analysis of mitochondrial morphology and number in microscopy images. Although these strategies are well suited for analysis of adhering cells with a flat morphology they are not appl

SUBMITTER: Nikolaisen J 

PROVIDER: S-EPMC4079598 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature